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Peer-Reviewed Publication
bioRxiv2026April 17, 2026Journal Article

SEC-seq reveals translation-focused metabolic strategies for high IgG productivity in clonal CHO cells.

Jasmine Tat1,2, Fides D Lay1, Jennitte Stevens1,3, Nathan E Lewis2,4,5
1Drug Substance Technologies, Amgen, Inc., Thousand Oaks, CA, USA.
2Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
3Present address: insitro Inc., South San Francisco, CA, USA.
4Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA.
5Center for Molecular Medicine, Complex Carbohydrate Research Center, and Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
PubMed PMC DOI PDF

Abstract

Chinese hamster ovary (CHO) cells are the dominant host for therapeutic protein production, yet intra- and inter-clonal heterogeneity in manufacturing phenotypes, and the underlying metabolic and secretory circuitry, remain poorly defined at single-cell resolution. Here, we apply secretion encoded single-cell sequencing (SEC-seq) to simultaneously measure transcriptomes and secreted IgG in single-…

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