Identifying Cardiac Autoantibody Biomarkers that Predict the Severity of Chagas Cardiomyopathy

Identifying Cardiac Autoantibody Biomarkers that Predict the Severity of Chagas Cardiomyopathy.

Heriks Ribeiro¹, Anton Dmitriev², Antonia Pereira Rosa Neta³, Meena Fatah⁴, Diptendu Chatterjee⁴, Lea Campos de Oliveira⁵, Fabiano Castro Albrecht⁶, Hui Tzu Lin Wang⁶, Juliana de Assis S Gomes⁷, Olgierd Woźniak⁸, Karolina Borowiec⁸, Elżbieta Katarzyna Biernacka⁸, Antonia Claudia Jácome da Câmara³, Luciana Sacilotto⁹, Francisco Carlos da Costa Darrieux⁹, Maurício Ibrahim Scanavacca⁹, José Eduardo Krieger¹⁰, Lúcia Maria da Cunha Galvão³, Walderez Dutra⁷, Antonio Luiz Pinho Ribeiro¹¹, Edecio Cunha-Neto¹², Maria Carmo Pereira Nunes¹¹, Ester Cerdeira Sabino⁵, Igor Cestari¹³, Andre Ducati Luchessi³, Vivian Nogueira Silbiger¹⁴, Robert M Hamilton¹⁵

¹Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, RN, Brazil; Department of Pediatrics, The Labatt Family Heart Centre and Translational Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada.

²Department of Pediatrics, The Labatt Family Heart Centre and Translational Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada; Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada.

³Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.

⁴Department of Pediatrics, The Labatt Family Heart Centre and Translational Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada.

⁵Department of Infectious Disease, Tropical Medicine, University of São Paulo, São Paulo, SP, Brazil.

⁶Dante Pazzanese Institute of Cardiology, São Paulo, SP, Brazil.

⁷Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.

⁸Department of Congenital Heart Diseases, Cardinal Stefan Wyszyński National Institute of Cardiology, Warsaw, Poland.

⁹Arrhythmia Unit, Heart Institute (Institute Coração), University of São Paulo Medical School, São Paulo, Brazil.

¹⁰Laboratory of Genetics and Molecular Cardiology (LGMC), Heart Institute (Institute Coração), University of São Paulo Medical School, São Paulo, Brazil.

¹¹Department of Internal Medicine, School of Medicine, Telehealth Center, Cardiology Service, Hospital das Clínicas, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

¹²Laboratory of Clinical Immunology and Allergy, Heart Institute (InCor), Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Institute of Investigation in Immunology, National Institute of Science and Technology (iii-INCT), São Paulo, Brazil.

¹³Institute of Parasitology, McGill University, Sainte-Anne-de-Bellevue, Quebec, Canada; Division of Clinical and Translational Research, Department of Medicine, McGill University, Montreal, Quebec, Canada.

¹⁴Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, RN, Brazil; Department of Pediatrics, The Labatt Family Heart Centre and Translational Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada. Electronic address: vivian.silbiger@sickkids.ca.

¹⁵Department of Pediatrics, The Labatt Family Heart Centre and Translational Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada. Electronic address: robert.hamilton@sickkids.ca.

Abstract

Chagas cardiomyopathy (ChCM) affects up to 30% of patients with chronic Chagas disease and lacks reliable biomarkers for severity and conduction abnormalities. We profiled cardiac autoantibodies using 2-dimensional gel electrophoresis and mass spectrometry, followed by enzyme-linked immunosorbent assay validation and machine learning analysis. Six autoantibodies linked to glycolysis, adenosine tri…

Abstract

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