Prospective metagenomic sequencing of wastewater across the United States yields robust viral enrichment and concordance with digital PCR measurements.

Marlene K Wolfe¹, Devin North¹, Alexander L Jaffe², Alessandro Zulli², Dorothea Duong³, Bridgette Shelden³, Miriam Goldman³, Miles Richardson³, Peter Thana³, Vikram Chan-Herur³, Pouya Kheradpour³, Amanda L Bidwell², Stephen P Hilton¹, Sheena Conforti², Abigail P Paulos¹, Alexandria B Boehm²

¹Gangarosa Department of Environmental Health, Emory University, Atlanta, Georgia, USA.

²Department of Civil and Environmental Engineering, Stanford University, Stanford, California, USA.

³Verily Health, South San Francisco, California, USA.

PubMed DOI

Abstract

Metagenomic sequencing is increasingly applied to wastewater to characterize the diversity, dynamics, and relative abundance of human and animal viruses. Among these sequencing approaches are those that enrich viral nucleic acids from the wastewater matrix, aiming to increase the viral read fraction for analysis. However, the feasibility of scaling targeted viral sequencing to diverse sewersheds a…

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