Peer-Reviewed Publication
bioRxiv2025December 7, 2025Journal Article
Overcoming the eIF2α Brake in Human Cell-Derived Translation Systems.
Nikolay A Aleksashin1,2, Rohan R Shelke2,3, Tianhao Yin2,4, Jamie H D Cate1,2,5
1Innovative Genomics Institute, University of California, Berkeley, CA, USA.
2Department of Molecular & Cell Biology, University of California, Berkeley, CA, USA.
3Present address: BillionToOne, 1035 O'Brien Dr, Menlo Park, CA 94025.
4Present address: Center of Molecular and Cellular Oncology, Yale Cancer Center at Yale University, 300 George Street, Suite 6400, New Haven, CT 06511.
5Department of Chemistry, University of California, Berkeley, CA, USA.
Abstract
Cell-free translation from human cells is a powerful platform for studying mammalian gene expression and building synthetic biology tools, but productivity is often curtailed by inhibitory phosphorylation of eIF2α on residue Ser52. Here we systematically explored complementary strategies to bypass this initiation block across editable and hard-to-edit human cell types. In Expi293F suspension cells…
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