Next-generation protein sequencing and individual ion mass spectrometry enable complementary analysis of interleukin-6.

Kenneth A Skinner¹, Troy D Fisher², Andrew Lee³, Taojunfeng Su⁴, Eleonora Forte⁵, Aniel Sanchez^2,6, Michael A Caldwell^2,7, Neil L Kelleher^8,9,10,11,12

¹Quantum-Si Incorporated, Branford, CT, USA.

²Proteomics Center of Excellence, Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.

³Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.

⁴Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA.

⁵Division of Nephrology, Department of Medicine, University of Illinois College of Medicine, Chicago, IL, USA.

⁶Department of Chemistry, Northwestern University, Evanston, IL, USA.

⁷Division of Hematology Oncology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.

⁸Proteomics Center of Excellence, Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA. n-kelleher@northwestern.edu.

⁹Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA. n-kelleher@northwestern.edu.

¹⁰Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA. n-kelleher@northwestern.edu.

¹¹Division of Hematology Oncology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA. n-kelleher@northwestern.edu.

¹²Department of Chemistry, Northwestern University, Evanston, IL, USA. n-kelleher@northwestern.edu.

PubMed PMC DOI PDF

Abstract

The vast complexity of the proteome currently overwhelms any single analytical technology in capturing the full spectrum of proteoform diversity. In this study, we evaluated the complementarity of two cutting-edge proteomic technologies-single-molecule protein sequencing and individual ion mass spectrometry-for analyzing recombinant human IL-6 (rhIL-6) at the amino acid, peptide, and intact proteo…

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