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Peer-Reviewed Publication
Methods Protoc2025;8(2)April 7, 2025Journal Article

Analysis of Processing, Post-Maturation, and By-Products of shRNA in Gene and Cell Therapy Applications.

Zhenyi Hong1, Nikola Tesic2, Xavier Bofill-De Ros1
1Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark.
2Seven Bridges Genomics Inc., Cambridge, MA 02138, USA.
PubMed PMC DOI PDF

Abstract

Short hairpin RNAs (shRNAs) are potent tools for gene silencing, offering therapeutic potential for gene and cell therapy applications. However, their efficacy and safety depend on precise processing by the RNA interference machinery and the generation of minimal by-products. In this protocol, we describe how to systematically analyze the processing of therapeutic small RNAs by DROSHA and DICER1 a…

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