Peer-Reviewed Publication
bioRxiv2025February 19, 2025Journal Article
High Dimensional Proteomic Multiplex Imaging of the Central Nervous System Using the COMET™ System.
Hinda Najem1,2, Sebastian Pacheco1,2, Jillyn Turunen1,2, Shashwat Tripathi1,2, Alicia Steffens1,3,2, Kathleen McCortney1,3,2, Jordain Walshon1,3,2, James Chandler1,2, Roger Stupp1,2, Maciej S Lesniak1,2, Craig M Horbinski1,3,2, Dan Winkowski4, Joanna Kowal5, Jared K Burks6, Amy B Heimberger1,2
1Department of Neurological Surgery, Northwestern University, Chicago, IL.
2Malnati Brain Tumor Institute of the Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago IL, 60611, USA.
3Department of Pathology Feinberg School of Medicine, Northwestern University, Chicago, IL.
4Visiopharm, Horsholm, Denmark.
5Lunaphore, Tolochenaz Switzerland, The University of Texas MD Anderson Cancer Center, Houston, Tx, 77030.
6Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, Tx, 77030.
Abstract
Sequential multiplex methodologies such as Akoya CODEX, Miltenyi MACSima, Rarecyte Orion, and others require modification of the antibodies by conjugation to an oligo or a specific fluorophore which means the use of off-the-shelf reagents is not possible. Modifications of these antibodies are typically performed via reduction chemistry and thus require verification and validation post-modification…
Create a free account to keep reading
Free members get 10 full research views every month across publications, clinical trials, FDA clearances, adverse events, and NIH grants. No credit card required.
Want unlimited research access? See Pro plans