Electrical stimulation of biofidelic engineered muscle enhances myotube size, force, fatigue resistance, and induces a fast-to-slow-phenotype shift.

Isabella Pallotta¹, Michael J Stec², Brian Schriver¹, David R Golann², Kevin Considine¹, Qi Su², Victor Barahona¹, Julia E Napolitano¹, Sarah Stanley¹, Meghan Garcia¹, Nicole T Feric¹, Krista M Durney¹, Roozbeh Aschar-Sobbi¹, Nathan Bays¹, Tea Shavlakadze², Michael P Graziano¹

¹Valo Health, Boston, Massachusetts, USA.

²Regeneron Pharmaceuticals, Tarrytown, New York, USA.

PubMed PMC DOI PDF

Abstract

Therapeutic development for skeletal muscle diseases is challenged by a lack of ex vivo models that recapitulate human muscle physiology. Here, we engineered 3D human skeletal muscle tissue in the Biowire II platform that could be maintained and electrically stimulated long-term. Increasing differentiation time enhanced myotube formation, modulated myogenic gene expression, and increased twitch an…

Create a free account to keep reading

Free members get 10 full research views every month across publications, clinical trials, FDA clearances, adverse events, and NIH grants. No credit card required.

Create Free Account Sign In

Want unlimited research access? See Pro plans

Data Accuracy Notice: Research intelligence on Health AI Central is aggregated from public sources (PubMed, ClinicalTrials.gov, FDA, NIH, CMS, and others) and refreshed nightly. Classifications and derived metrics are produced by automated methods described in our Methodology. We recommend verifying critical data points against the primary sources before making decisions.