Health AI Central
Peer-Reviewed Publication
BMC Bioinformatics2022;23(1):74.February 16, 2022Journal Article

CROPSR: an automated platform for complex genome-wide CRISPR gRNA design and validation.

Hans Müller Paul1,2, Dave D Istanto2,3,4, Jacob Heldenbrand5,6, Matthew E Hudson7,8,9,10
1Illinois Informatics Institute, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
2Center for Advanced Bioenergy and Bioproducts Innovation, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
3Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
4OmniTier Inc., Milpitas, CA, USA.
5National Center for Supercomputer Applications, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
6ClosedLoop.ai, Round Rock, TX, USA.
7Illinois Informatics Institute, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. mhudson@illinois.edu.
8Center for Advanced Bioenergy and Bioproducts Innovation, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. mhudson@illinois.edu.
9Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. mhudson@illinois.edu.
10National Center for Supercomputer Applications, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. mhudson@illinois.edu.
PubMed PMC DOI PDF

Abstract

BACKGROUND: CRISPR/Cas9 technology has become an important tool to generate targeted, highly specific genome mutations. The technology has great potential for crop improvement, as crop genomes are tailored to optimize specific traits over generations of breeding. Many crops have highly complex and polyploid genomes, particularly those used for bioenergy or bioproducts. The majority of tools curren…

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