Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded Adeno-Associated Viral Genomes in Academic Settings.

Lucy H Maynard¹, Olivia Smith¹, Nicolas P Tilmans², Eleonore Tham¹, Shayan Hosseinzadeh¹, Weilun Tan¹, Ryan Leenay¹, Andrew P May¹, Nicole K Paulk^3,4

¹Chan Zuckerberg Biohub, Department of Genome Engineering, San Francisco, California.

²Anagenex, San Francisco, California.

³Genome Engineering, Chan Zuckerberg Biohub, San Francisco, California.

⁴Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California.

PubMed PMC DOI PDF

Abstract

Adeno-associated viral (AAV) vectors have shown great promise in gene delivery as evidenced by recent FDA approvals. Despite efforts to optimize manufacturing for good manufacturing practice (GMP) productions, few academic laboratories have the resources to assess vector composition. One critical component of vector quality is packaged genome fidelity. Errors in viral genome replication and packag…

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