Dendrimer-like polymeric DNAs as chemiluminescence probes for amplified detection of telomere DNA on a solid-phase membrane.

Ahmed F M El-Mahdy¹, Takayuki Shibata, Tsutomu Kabashima, Masaaki Kai

¹Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8521, Japan. ms-kai@nagasaki-u.ac.jp.

PubMed DOI

Abstract

For the first time, an amplified chemiluminescence (CL) detection of the telomere DNA spotted on a nylon membrane is described here, based on the direct hybridization with the CL probe of dendrimer-like polymeric DNAs possessing a large number of guanine moieties. This probe was synthesized by sense and antisense hybridization between Y-shaped DNAs and then could hybridize with the target DNA.

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