Sensitive determination of a beta-lactam antibiotic, cefaclor by liquid chromatography with chemiluminescence detection.

Masaaki Kai¹, Hiromi Kinoshita, Kazuko Ohta, Shuuji Hara, Myung Koo Lee, Jianzhong Lu

¹School of Pharmaceutical Sciences, Nagasaki University, Bunkyo-Machi 1-14, Nagasaki 852-8521, Japan. ms-kai@net.nagasaki-u.ac.jp

PubMed DOI

Abstract

We present a sensitive method for the determination of cefaclor (CCL), a beta-lactam antibiotic. It was based on the chemical derivatization of the drug with 4-(2'-cyanoisoindolyl)phenylisothiocynate (CIPIC) under the reaction conditions with heating at 80 degrees C for 7 min in the presence of pyridine. The CIPIC reagent could react with the primary amino group of the drug to form the CIPIC-conju…

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