Peer-Reviewed Publication
iScience2026;29(10):117654.October 16, 2026Journal Article
Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine.
Foteini Tzioufa1, Merve Ceylan1, Maria Letizia Di Martino2, Dinh Son Vo1, Ana C C Lopes2, Rebekkah Hammar1, Magnus Sundbom3, Martin Skogar3, Per M Hellström4, Dominic-Luc Webb4,5, Maria Karlgren1, Iain Gardner6, Patrik Lundquist1, Jens Eriksson2, Daisy Hjelmqvist1, Mikael E Sellin2,7, Madlen Hubert1, Per Artursson1
1Department of Pharmacy, Uppsala University, 752 37 Uppsala, Sweden.
2Department of Medical Biochemistry and Microbiology, Uppsala University, 752 37 Uppsala, Sweden.
3Department of Surgical Sciences, Uppsala University, 751 85 Uppsala, Sweden.
4Department of Medical Sciences, Uppsala University, 751 85 Uppsala, Sweden.
5DLW Bioanalytics, 195 33 Märsta, Sweden.
6Certara UK Ltd, Simcyp Division, Level 2-Acero, Sheffield S1 2BJ, UK.
7Science for Life Laboratory, 752 37 Uppsala, Sweden.
Abstract
Intestinal enteroids are "mini-guts" that recapitulate essential features of the intestinal epithelium in vitro. We developed and characterized native basal-out (BO) and morphogenically everted apical-out (AO) jejunal enteroids cultured in suspension. Using quantitative proteomics and a range of advanced microscopy techniques, we show that these enteroids progressively adopt protein profiles close…
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