Peer-Reviewed Publication
Methods Mol Biol2026;3036387-407.January 1, 2026Journal Article

Image Analysis Approaches for In Situ Hybridization Technologies.

David Mason1, Julia Jones2
1Visiopharm A/S, Agern Allé 24, 2970, Hoersholm, Denmark. dma@visiopharm.com.
2Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.

Abstract

In situ hybridisation (ISH) is an invaluable experimental technique when considering the holistic process of transcription through to translation. Without good tools to identify sequence-based structures within cells and tissues, researchers are left with only half of the story when looking at protein expression. While there are many experimental approaches, including a rainbow of chromogens and a…

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