A simplified hybrid capture approach retains high specificity and enables PCR-free workflow.

Adeline Huizhen Mah¹, Xiaodong Qi¹, Junhua Zhao¹, Kelly Wiseman¹, Laure Edoli¹, Kyle Metcalfe¹, Kyle Donohoe¹, Micah Ojeda¹, Sophie Billings¹, Juan Moreno¹, Marina McCowin¹, Ben Krajacich¹, Kevin Green¹, Ramkrishna Adhikary¹, Andrew Boddicker¹, Joshua Chan¹, Peter Mains¹, Bryan Lajoie¹, Sean Devitt¹, Semyon Kruglyak¹, Shawn Levy¹, Michael Previte²

¹Element Biosciences, San Diego, CA, USA.

²Element Biosciences, San Diego, CA, USA. mprevite@elembio.com.

PubMed PMC DOI PDF

Abstract

BACKGROUND: Hybrid capture is a critical technology for selective enrichment of genomic regions of interest in genomic analysis. Despite its widespread adoption, the core methodology has remained largely unchanged for over 15 years, with traditional workflows involving time-consuming bead-based capture steps, multiple temperature-controlled washes, and post-hybridization PCR. These steps introduce…

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