Peer-Reviewed Publication
bioRxiv2025February 8, 2025Journal Article
Next-Generation Protein Sequencing and individual ion mass spectrometry enable complementary analysis of interleukin-6.
Kenneth A Skinner1, Troy D Fisher2, Andrew Lee3, Taojunfeng Su3, Eleonora Forte2,4, Aniel Sanchez2, Michael A Caldwell2,5, Neil L Kelleher2,3,6
1Quantum-Si, Incorporated, Branford, Connecticut, United States.
2Proteomics Center of Excellence, Northwestern University, Evanston, Illinois, United States.
3Departments of Molecular Biosciences, Chemistry and Chemical and Biological Engineering, Northwestern University, Evanston, IL, United States.
4Department of Surgery, Feinberg School of Medicine, Comprehensive Transplant Center, Northwestern University, Chicago, Illinois, United States.
5Department of Medicine, Division of Hematology Oncology, Feinberg School of Medicine, Northwestern University, Chicago, IL, United States.
6Department of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, IL, United States.
Abstract
The vast complexity of the proteome currently overwhelms any single analytical technology in capturing the full spectrum of proteoform diversity. In this study, we evaluated the complementarity of two cutting-edge proteomic technologies-single-molecule protein sequencing and individual ion mass spectrometry-for analyzing recombinant human IL-6 (rhIL-6) at the amino acid, peptide, and intact proteo…
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