Peer-Reviewed Publication
bioRxiv2024October 9, 2024Journal Article
Molecular model of a bacterial flagellar motor in situ reveals a "parts-list" of protein adaptations to increase torque.
Tina Drobnič1,2, Eli J Cohen1, Tom Calcraft3, Mona Alzheimer4, Kathrin Froschauer4, Sarah Svensson4,5, William H Hoffmann6, Nanki Singh1, Sriram G Garg7, Louie Henderson1,8, Trishant R Umrekar1, Andrea Nans9, Deborah Ribardo10, Francesco Pedaci6, Ashley L Nord6, Georg K A Hochberg7, David R Hendrixson10, Cynthia M Sharma4, Peter B Rosenthal3, Morgan Beeby1
1Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK.
2Tina Drobnič current affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.
3Structural Biology of Cells and Viruses Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
4University of Würzburg, Institute of Molecular Infection Biology, Department of Molecular Infection Biology II, Josef-Schneider-Straße 2/D15, 97080 Würzburg, Germany.
5Current affiliation: The Center for Microbes, Development and Health, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China 200031. email: sarah.svensson@ips.ac.cn.
6Centre de Biologie Structurale, Universite de Montpellier, CNRS, INSERM. Montpellier, France.
7Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
8Current affiliation: Peptone Ltd, 370 Grays Inn Road, London WC1X 8BB, UK.
9Structural Biology Science Technology Platform, The Francis Crick Institute, London NW1 1AT, UK.
10Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390.
Abstract
One hurdle to understanding how molecular machines work, and how they evolve, is our inability to see their structures in situ. Here we describe a minicell system that enables in situ cryogenic electron microscopy imaging and single particle analysis to investigate the structure of an iconic molecular machine, the bacterial flagellar motor, which spins a helical propeller for propulsion. We determ…
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