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Peer-Reviewed Publication
PeerJ2024;12e18100.January 1, 2024Journal Article

Identifying transgene insertions in Caenorhabditis elegans genomes with Oxford Nanopore sequencing.

Paula E Adams1,2, Jennifer L Thies2,3, John M Sutton2,4, Joshua D Millwood2,5, Guy A Caldwell2, Kim A Caldwell2, Janna L Fierst6,7
1Department of Biological Sciences, Auburn University, Auburn, AL, United States of America.
2Department of Biological Sciences, University of Alabama - Tuscaloosa, Tuscaloosa, AL, United States of America.
3Curriculum in Toxicology and Environmental Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
4Absci, Vancouver, WA, United States of America.
5Department of Biological and Environmental Sciences, University of West Alabama, Livingston, AL, United States of America.
6Department of Biological Sciences, Florida International University, Miami, FL, United States of America.
7Biomolecular Sciences Institute, Florida International University, Miami, FL, United States of America.
PubMed PMC DOI PDF

Abstract

Genetically modified organisms are commonly used in disease research and agriculture but the precise genomic alterations underlying transgenic mutations are often unknown. The position and characteristics of transgenes, including the number of independent insertions, influences the expression of both transgenic and wild-type sequences. We used long-read, Oxford Nanopore Technologies (ONT) to seque…

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