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Peer-Reviewed Publication
bioRxiv2025January 27, 2025Journal Article

Integrated transcriptomic analysis of human induced pluripotent stem cell-derived osteogenic differentiation reveals a regulatory role of KLF16.

Ying Ru1, Meng Ma2, Xianxiao Zhou1,3,4, Divya Kriti1,5, Ninette Cohen1,6, Sunita D'Souza7,8, Christoph Schaniel7,9,10,11, Susan M Motch Perrine12, Sharon Kuo13,14, Oksana Pichurin15, Dalila Pinto1, Genevieve Housman16,17, Greg Holmes1, Eric Schadt1,4, Harm van Bakel1,4,18, Bin Zhang1,3,4, Ethylin Wang Jabs1,7,15,19, Meng Wu1,15,19
1Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA.
2Mount Sinai Genomics, Sema4, Stamford, CT, 06902, USA.
3Mount Sinai Center for Transformative Disease Modeling, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA.
4Icahn Genomics Institute, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA.
5Present address: Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of British Columbia, Vancouver, BC V6T 2G3, Canada.
6Present address: Division of Cytogenetics and Molecular Pathology, Zucker School of Medicine at Hofstra/Northwell, Northwell Health Laboratories, Lake Success, NY, 11030, USA.
7Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA.
8Present address: St Jude Children's Research Hospital, Memphis, TN, 38105, USA.
9Department of Medicine, Division of Hematology and Medical Oncology, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA.
10Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA.
11Mount Sinai Institute for Systems Biomedicine, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA.
12Department of Anthropology, Pennsylvania State University, University Park, PA, 16802, USA.
13Department of Biomedical Sciences, University of Minnesota, Duluth, MN, 55812, USA.
14Technological Primates Research Group, Max Planck Institute for Evolutionary Anthropology, Leipzig, 04103, Germany.
15Department of Clinical Genomics, Mayo Clinic, Rochester, MN, 55905, USA.
16Section of Genetic Medicine, Department of Medicine, University of Chicago, Chicago, IL, 60637, USA.
17Department of Primate Behavior and Evolution, Max Planck Institute for Evolutionary Anthropology, Leipzig, 04103, Germany.
18Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA.
19Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, 55905, USA.
PubMed PMC DOI PDF

Abstract

Osteogenic differentiation is essential for bone development, metabolism, and repair; however, the underlying regulatory relationships among genes remain poorly understood. To elucidate the transcriptomic changes and identify novel regulatory genes involved in osteogenic differentiation, we differentiated mesenchymal stem cells (MSCs) derived from 20 human iPSC lines into preosteoblasts (preOBs) a…

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