Peer-Reviewed Publication
Curr Opin Biotechnol2023;82102953.August 1, 2023Journal Article
The cofactor challenge in synthetic methylotrophy: bioengineering and industrial applications.
Jan L Krüsemann1, Vittorio Rainaldi2, Charles Ar Cotton3, Nico J Claassens2, Steffen N Lindner4
1Charité - Universitätsmedizin Berlin, Department of Biochemistry, Charitéplatz 1, 10117 Berlin, Germany; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, Germany; Max Planck Institute for Terrestrial Microbiology, Department of Biochemistry and Synthetic Metabolism, Karl-von-Frisch-Str. 10, 35043 Marburg, Germany.
2Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands.
3Cambrium GmbH, Max-Urich-Straße 3, 13355 Berlin, Germany.
4Charité - Universitätsmedizin Berlin, Department of Biochemistry, Charitéplatz 1, 10117 Berlin, Germany; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, Germany. Electronic address: steffen.lindner@charite.de.
Abstract
Methanol is a promising feedstock for industrial bioproduction: it can be produced renewably and has high solubility and limited microbial toxicity. One of the key challenges for its bio-industrial application is the first enzymatic oxidation step to formaldehyde. This reaction is catalysed by methanol dehydrogenases (MDH) that can use NAD+, O2 or pyrroloquinoline quinone (PQQ) as an electron acce…
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