Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development.

Hanqing Li¹, Ash Watson^2,3, Agnieszka Olechwier⁴, Michael Anaya¹, Siamak K Sorooshyari⁵, Dermott P Harnett^2,3, Hyung-Kook Peter Lee¹, Jost Vielmetter¹, Mario A Fares^2,6, K Christopher Garcia^7,8, Engin Özkan⁴, Juan-Pablo Labrador^2,3, Kai Zinn¹

¹Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States.

²Smurfit Institute of Genetics, Trinity College Dublin, Dublin, Ireland.

³Institute of Neuroscience, Trinity College Dublin, University of Dublin, Dublin, Ireland.

⁴Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, United States.

⁵Ellipsis Health, San Francisco, United States.

⁶Department of Abiotic Stress, Group of Integrative and Systems Biology, Instituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politécnica de Valencia), Valencia, Spain.

⁷Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, United States.

⁸Department of Structural Biology, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, United States.

PubMed PMC DOI PDF

Abstract

An 'interactome' screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification…

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Mentioned Companies

Ellipsis Health
affiliation

Other Funding

NINDS NIH HHS(R01 NS097161)
United States
NINDS NIH HHS(R37 NS028182)
United States

MeSH Terms

Animals0Antibodies0Biological Assay0Computational Biology0Drosophila Proteins0Drosophila melanogaster0Embryo, Nonmammalian0Fluorescent Dyes0Gene Expression Regulation, Developmental0Growth Cones0Membrane Proteins0Motor Neurons0Muscles0Nerve Tissue Proteins0Nervous System0Phycoerythrin0Phylogeny0Protein Interaction Mapping0Protein Isoforms0Signal Transduction0

Keywords

D. melanogasterimmunglobulin superfamilyinteractomemotor axon guidancemotor neuronneural developmentneuroscienceprotein-protein interaction

Article Info

PMID: 28829740
PMCID: PMC5578738
DOI: 10.7554/eLife.28111
Received: April 27, 2017
Accepted: July 28, 2017

How to Cite

Li H et al. Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development. Elife2017;6 doi:10.7554/eLife.28111

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