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Peer-Reviewed Publication
Biotechniques2003;35(1):78-80, 82-3.July 1, 2003Evaluation Study

Dictyostelium discoideum transformation by oscillating electric field electroporation.

Laeticia Alibaud1, Pierre Cosson, Mohammed Benghezal
1Université de Genève, Athelas SA, Geneva, Switzerland.
PubMed DOI

Abstract

Dictyostelium discoideum has been used as a genetically tractable model organism to study many biological phenomena. High-efficiency transformation is a prerequisite for successful genetic screens such as mutant complementation, identification of suppressor genes, or insertional mutagenesis. Although exponential decay electroporation is the standard transformation technique for D. discoideum, its…

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